Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Yield and intercellular distribution of dicentric chromosomal aberrations after in vitro X-rays irradiation of blood samples derived from four Saudi individuals

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: In Vitro, Irradiation, Derivative Assay

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Linear-quadratic dose–response curves ( solid lines ) for dicentric chromosomal aberrations induced by 320 keV X-rays in lymphocytes derived from four Saudi volunteers. Data points represent the yield of dicentric per metaphase scored using manually assisted mode. Broken lines are the 95 % confidence limits calculated assuming Poisson distribution. Error bars represent the standard errors

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: Derivative Assay

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Yield and intercellular distribution of dicentric chromosomal aberrations induced in peripheral blood lymphocytes by X-rays exposure

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques:

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Representative example of metaphase with dicentric chromosome captured by the Metafer4 system ( upper panel ) and normal metaphase in control (0 Gy) and dicentric ( arrow ), acentric fragments ( squared arrow head ) and rings ( rounded arrow head ) in irradiated lymphocytes ( lower panel )

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: Control, Irradiation

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Linear-quadratic dose–response calibration curves ( solid lines ) for dicentric chromosomal aberrations induced by 320 keV X-rays in lymphocytes derived from four Saudi volunteers. Data points represent the yield of dicentric per metaphase scored using either manually assisted ( circles ) or automated ( diamonds ) mode. Broken lines are the 95 % confidence limits calculated assuming Poisson distribution. Error bars represent the standard errors

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: Derivative Assay

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Illustrative practical example of estimating radiation dose received, with its 95 % confidence interval, in a scenario of total body irradiation using CABAS software. Utilizing the coefficients of the national calibration curve, a hypothetical accidental over-exposure that yields, for example, 150 dicentric per 280 metaphases, would be caused by an absorbed dose of 2.416 Gy with a lower and upper 95 % confidence limits of 2.21 and 2.63 Gy, respectively

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: Irradiation, Software

Journal: 3 Biotech

Article Title: Establishing cytogenetic biodosimetry laboratory in Saudi Arabia and producing preliminary calibration curve of dicentric chromosomes as biomarker for medical dose estimation in response to radiation emergencies

doi: 10.1007/s13205-014-0217-x

Figure Lengend Snippet: Metafer automated scoring of dicentric chromosomal aberrations induced in vitro in human lymphocytes by X-rays exposure

Article Snippet: Comparing the coefficients of the dose–response relationship for dicentric induction ( Y = 0.0017 + 0.0260 × D + 0.0806 × D 2 ) with those from similar published studies (Schmid et al. ; Barquinero et al. ; IAEA ; Beinke et al. ), good general agreement can be observed.

Techniques: In Vitro